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KMID : 0545119990090060778
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Journal of Microbiology and Biotechnology
1999 Volume.9 No. 6 p.778 ~ p.783
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Characterization of Campylobacters Newly Isolated from Swine Gastric Mucosa
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Lee, Yeon Hee
Lee, Jin Hui/Cho, Hyun Joo/Shin, En Joo/Park, Jong Hwan/Park, Jae Hak
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Abstract
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Campylobacter is a pathogen for both humans and animals that can be transferred from animals to humans. Four isolates, which grew under 5-10% CO_2 and had small and translucent colonies, were obtained from swine gastric mucosa and characterized using various methods. These bacteria were gram negative, spirally shaped with round ends. One or two non-sheathed polar flagella were observed under electron microscopy. A PCR with species-specific protein (SSP) primers for 16S rRNA gene in Campylobacter produced a typical 462bp fragment. The isolates had various biochemical and molecular characteristics which differentiated them from other Campylobacters. The isolates were catalase and oxidase positive, urease (rapid) negative, nitrate reduction positive, indoxyl acetate hydrolysis positive, γ-glutamyl transpeptidase negative, and alkaline phosphatase negative. All four isolates showed growth at 37℃ and 42℃ but not at 25℃, were resistant to cephalotin and cefoperazone, and susceptible to carbenicillin. The isolates showed various results in the reduction of chloride to triphenyl tetrazolium (TTC) and a susceptibility to nalidixic acid. Western blot analysis of these isolates with antiserum raised against one isolate showed different patterns from those of reference strains. A dendrogram drawn with the RAPD results showed that these isolates belonged to a new Campylobacter spp. group different from those of C. jejuni, C. doylei, C. lari, and C. coli.
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